Genomewide Identification and Expression Analyses of <i>PAL</i> Genes in Different Color Radish

Phenylalanine ammonia-lyase (PAL) is the first step key enzyme of flavonoid biosynthesis pathway which played an essential role in plant anthocyanin accumulation. Five PAL family gene members were identified radish ( Raphanus sativus  L.) genome name RsPAL1~5. Specific primers designed to amplify open reading frame red ‘Hongxin No.1’ and then sequenced. Real-time PCR was used analysis expression pattern 5  RsPALs  in four different tissues including leaf, petiole, taproot flesh skin form five color type radishes, ‘Shaguan’ No.1’, white ‘Shaguan ‘Mantanghong’, ‘Chunbulao’. The results showed that length read RsPAL1~5 2 160, 166, 163, 124 109 bp encoding for 719, 721, 720, 707 702 amino acid residues respectively. Sequence alignment MIO motif (Ala-Gly-Ser) conserved among proteins. RsPAL1~4 clustered with Arabidopsis AtPAL1 AtPAL2, RsPAL5 AtPAL4 phylogenetic tree. suggested that  RsPAL4  was expressed only tissue accumulate anthocyanin,  RsPAL4 significantly correlated content. These indicating  may specifically involved radish. However, no obvious other   members found this study, suggesting they may participate other secondary metabolites phenylpropane metabolism pathway. This study would provide scientific basis further on function radish  .